Journal: Molecules

Article Title: New Biscoumarin Derivatives: Synthesis, Crystal Structure, Theoretical Study and Antibacterial Activity against Staphylococcus aureus

doi: 10.3390/molecules191219868

Figure Lengend Snippet: MIC of compounds 1 – 5 and antibiotics in Mueller-Hinton Broth Culture.

Article Snippet: Four S. aureus bacterial strains, including one drug-sensitive S. aureus ( S. aureus ATCC 29213) strain and three MRSA strains (MRSA XJ 75302, Mu50, USA 300 LAC), were used in the systematic analysis of the antibacterial activities of compounds 1 – 5 in vitro .

Techniques:

Journal: Molecules

Article Title: New Biscoumarin Derivatives: Synthesis, Crystal Structure, Theoretical Study and Antibacterial Activity against Staphylococcus aureus

doi: 10.3390/molecules191219868

Figure Lengend Snippet: Concentration-dependent inhibition of compound 1 on the growth of S. aureus ATCC 29213, MRSA XJ 75302, Mu50, and MRSA USA 300 LAC. The cells were cultured in liquid culture medium and treated with different concentrations of compound 1 .

Article Snippet: Four S. aureus bacterial strains, including one drug-sensitive S. aureus ( S. aureus ATCC 29213) strain and three MRSA strains (MRSA XJ 75302, Mu50, USA 300 LAC), were used in the systematic analysis of the antibacterial activities of compounds 1 – 5 in vitro .

Techniques: Concentration Assay, Inhibition, Cell Culture

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Antioxidant activity and antibacterial evaluation of new thiazolin-4-one derivatives as potential tryptophanyl-tRNA synthetase inhibitors

doi: 10.1080/14756366.2019.1596086

Figure Lengend Snippet: The top binding affinity of compounds to E. coli TrpRS and S. aureus TrpRS, the computed inhibition constant and the mean binding affinities of compounds’ conformations in the 2 Å cluster.

Article Snippet: There are many validated primary structures of TrpRS isolated from various strains of S. aureus deposited in UniProt: P67593 – (strain N315), P67592 – strain Mu50/ATCC 700699, Q5HH88 – strain COL, Q6GI89 – strain MRSA252, Q6GAT0 – strain MSSA476, P67594 – strain MW2, T1Y8L7 from S. aureus subsp. aureus CN1, A0A0E0VMI8 from S. aureus subsp. aureus 71193, A0A0E1AGS6 from S. aureus subsp. aureus Z172, Q2FZQ7 – strain NCTC 8325, A0A0E1VIN1 from S. aureus subsp. aureus USA300_TCH959, A0A0E1X8M2 from S. aureus subsp. aureus MN8, A0A0H3KFE9 – strain Newman, A0A0H2XGX6 – strain USA300, A0A2H5AMI2 - strain 046.

Techniques: Binding Assay, Inhibition

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Antioxidant activity and antibacterial evaluation of new thiazolin-4-one derivatives as potential tryptophanyl-tRNA synthetase inhibitors

doi: 10.1080/14756366.2019.1596086

Figure Lengend Snippet: Polar contacts made by the top binding conformation of each compound to the catalytic site of TrpRS.

Article Snippet: There are many validated primary structures of TrpRS isolated from various strains of S. aureus deposited in UniProt: P67593 – (strain N315), P67592 – strain Mu50/ATCC 700699, Q5HH88 – strain COL, Q6GI89 – strain MRSA252, Q6GAT0 – strain MSSA476, P67594 – strain MW2, T1Y8L7 from S. aureus subsp. aureus CN1, A0A0E0VMI8 from S. aureus subsp. aureus 71193, A0A0E1AGS6 from S. aureus subsp. aureus Z172, Q2FZQ7 – strain NCTC 8325, A0A0E1VIN1 from S. aureus subsp. aureus USA300_TCH959, A0A0E1X8M2 from S. aureus subsp. aureus MN8, A0A0H3KFE9 – strain Newman, A0A0H2XGX6 – strain USA300, A0A2H5AMI2 - strain 046.

Techniques: Binding Assay, Residue

Journal: International immunopharmacology

Article Title: Immunomodulatory effects of recombinant lactoferrin during MRSA infection

doi: 10.1016/j.intimp.2014.02.029

Figure Lengend Snippet: BALB/c mice were infected IP with MRSA (LD100) were treated at 2 hours post-infection with rmLF (100µg/mouse). Survival was monitored post-infection. Control (non-treated) mice succumbed to MRSA infection within 24 hours. Mice treated with rmLF demonstrated increased survival. Data representative of two experimental repeats with 8 mice per experiment.

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) Mu50 strain (ATCC) with intermediate vancomycin resistance was grown in BBLTM Muller-Hinton (MH) (Becton, Dickinson and Company) broth supplemented with 4μg/mL of Vancomycin (according to Sigma protocols) at 37°C and rotating at 225rpm.

Techniques: Infection, Control

Journal: International immunopharmacology

Article Title: Immunomodulatory effects of recombinant lactoferrin during MRSA infection

doi: 10.1016/j.intimp.2014.02.029

Figure Lengend Snippet: BALB/c mice were infected with MRSA (LD100) and treated with 100µg/mouse rmLF at 2 hours post-infection. Blood was collected 1 hour later and colony forming units (CFU) were enumerated. Mice treated with rmLF showed a slight, but non-significant, decrease compared to non-treated controls. Data graphed as mean +/− standard deviation, log scale. Data representative of three experimental repeats with 4–5 mice per experiment.

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) Mu50 strain (ATCC) with intermediate vancomycin resistance was grown in BBLTM Muller-Hinton (MH) (Becton, Dickinson and Company) broth supplemented with 4μg/mL of Vancomycin (according to Sigma protocols) at 37°C and rotating at 225rpm.

Techniques: Infection, Standard Deviation

Journal: International immunopharmacology

Article Title: Immunomodulatory effects of recombinant lactoferrin during MRSA infection

doi: 10.1016/j.intimp.2014.02.029

Figure Lengend Snippet: MRSA-infected mice given rmLF as described in Figures 1 and ​and22 demonstrated significant decrease in IL-17, but not IL-6. Data representative of three experimental repeats with at least 5 mice per experiment; rmLF alone did not induce responses above background levels. N=8 depicted with mean +/− standard deviation.

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) Mu50 strain (ATCC) with intermediate vancomycin resistance was grown in BBLTM Muller-Hinton (MH) (Becton, Dickinson and Company) broth supplemented with 4μg/mL of Vancomycin (according to Sigma protocols) at 37°C and rotating at 225rpm.

Techniques: Infection, Standard Deviation

Journal: International immunopharmacology

Article Title: Immunomodulatory effects of recombinant lactoferrin during MRSA infection

doi: 10.1016/j.intimp.2014.02.029

Figure Lengend Snippet: Modulation of pro-inflammatory cytokine production from MRSA-infected mouse splenocytes treated with rmLF.

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) Mu50 strain (ATCC) with intermediate vancomycin resistance was grown in BBLTM Muller-Hinton (MH) (Becton, Dickinson and Company) broth supplemented with 4μg/mL of Vancomycin (according to Sigma protocols) at 37°C and rotating at 225rpm.

Techniques:

Journal: International immunopharmacology

Article Title: Immunomodulatory effects of recombinant lactoferrin during MRSA infection

doi: 10.1016/j.intimp.2014.02.029

Figure Lengend Snippet: TNF-α production from MRSA infected granulocytes after rmouseLF treatment.

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) Mu50 strain (ATCC) with intermediate vancomycin resistance was grown in BBLTM Muller-Hinton (MH) (Becton, Dickinson and Company) broth supplemented with 4μg/mL of Vancomycin (according to Sigma protocols) at 37°C and rotating at 225rpm.

Techniques: Infection

Journal: International immunopharmacology

Article Title: Immunomodulatory effects of recombinant lactoferrin during MRSA infection

doi: 10.1016/j.intimp.2014.02.029

Figure Lengend Snippet: Human blood monocytes isolated from healthy human buffy coats were infected with MRSA (MOI 1:10) and treated with rhLF at 2 hours post-infected were analyzed for cytokine production at 6 hours post-treatment. Addition of rhLF at 100µg/mL increased MRSA-infected PBMC production of IFN-γ and IL-2. rhLF alone did not induce responses above background levels. Data represented as box and whiskers with the box expressing mean, minimum, and maximum, and whiskers as standard deviation. ***=p<0.001

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) Mu50 strain (ATCC) with intermediate vancomycin resistance was grown in BBLTM Muller-Hinton (MH) (Becton, Dickinson and Company) broth supplemented with 4μg/mL of Vancomycin (according to Sigma protocols) at 37°C and rotating at 225rpm.

Techniques: Isolation, Infection, Expressing, Standard Deviation

Journal: International immunopharmacology

Article Title: Immunomodulatory effects of recombinant lactoferrin during MRSA infection

doi: 10.1016/j.intimp.2014.02.029

Figure Lengend Snippet: Human blood monocytes isolated from healthy human buffy coats were infected with MRSA (MOI 1:10) and treated with rhLF at 2 hours postinfected were analyzed for cytokine production at 6 hours post-treatment. Treatment with rhumanLF decreased levels of IL-6, IL-1β, and IL-12p40. A non-significant decrease was also observed in IL-10. *=p<0.05; **=p<0.01; ***=p<0.001

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) Mu50 strain (ATCC) with intermediate vancomycin resistance was grown in BBLTM Muller-Hinton (MH) (Becton, Dickinson and Company) broth supplemented with 4μg/mL of Vancomycin (according to Sigma protocols) at 37°C and rotating at 225rpm.

Techniques: Isolation, Infection

Journal: International immunopharmacology

Article Title: Immunomodulatory effects of recombinant lactoferrin during MRSA infection

doi: 10.1016/j.intimp.2014.02.029

Figure Lengend Snippet: Granulocytes isolated from human buffy coats were infected with MRSA (MOI 1:10) and treated with rhLF at 2 hours post-infection. Supernatants were collected and analyzed by ELISA at 6 hours post-treatment. While there was no change in TNF-α, addition of rhLF led to an increase in IL-1β production from MRSA-infected granulocytes. Data represented as box and whiskers with the box expressing mean, minimum, and maximum, and whiskers as standard deviation. *=p<0.05

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) Mu50 strain (ATCC) with intermediate vancomycin resistance was grown in BBLTM Muller-Hinton (MH) (Becton, Dickinson and Company) broth supplemented with 4μg/mL of Vancomycin (according to Sigma protocols) at 37°C and rotating at 225rpm.

Techniques: Isolation, Infection, Enzyme-linked Immunosorbent Assay, Expressing, Standard Deviation